Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia
نویسندگان
چکیده
BACKGROUND The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA). METHODS Canine (n = 247) and feline (n = 74) blood samples were submitted for DAT testing to two laboratories. A subset of canine samples was categorized as having idiopathic IMHA, secondary IMHA, or no IMHA. RESULTS The kappa values for agreement between the tests were in one laboratory 0.86 for canine and 0.58 for feline samples, and in the other 0.48 for canine samples. The lower agreement in the second laboratory was caused by a high number of positive canine DATs for which the gel test was negative. This group included significantly more dogs with secondary IMHA. CONCLUSIONS The gel test might be used as a screening test for idiopathic IMHA and is less often positive in secondary IMHA than the DAT.
منابع مشابه
Efficacy of Microtiter Method of Direct Antiglobulin Test in diagnosis of Immune Mediated Haemolytic Anaemia in Dogs
The present study was carried out to assess the efficacy of microtiter method of direct antiglobulin test in diagnosis of Immune Mediated Haemolytic Anaemia in Dogs. Two hundred and fifty eight anaemic dogs with pale/icteric mucous membrane were used for the present study. Initial screening of these dogs was done with saline agglutination test. Forty seven dogs were found positive for saline ag...
متن کاملThe use of the rapid osmotic fragility test as an additional test to diagnose canine immune-mediated haemolytic anaemia
BACKGROUND Diagnosing canine immune-mediated haemolytic anaemia (IMHA) is often challenging because all currently available tests have their limitations. Dogs with IMHA often have an increased erythrocyte osmotic fragility (OF), a characteristic that is sometimes used in the diagnosis of IMHA. Since the classic osmotic fragility test (COFT) is time-consuming and requires specialized equipment, ...
متن کاملManagement of cold haemolytic syndrome.
Most haemolytic disease is mediated by immunoglobulin G (IgG) antibodies and leads to red blood cell destruction outside of the circulatory system. However, rare syndromes, such as paroxysmal cold haemoglobinuria, show IgG antibodies causing intravascular destruction. Haemolysis may also occur because of immunoglobulin M antibodies. Historically, these antibodies have been termed 'cold agglutin...
متن کاملTuberculosis induced autoimmune haemolytic anaemia: a systematic review to find out common clinical presentations, investigation findings and the treatment options
Background Tuberculosis induced autoimmune haemolytic anaemia is a rare entity. The aim of this study was to explore its common presentations, investigation findings and treatment options through a systematic review of published reports. Methods PubMed, Trip, Google Scholar, Science Direct, Cochrane Library, Open-Grey, Grey literature report and the reference lists of the selected articles we...
متن کاملQualitative and Semi-Quantitative Comparison of an Rk39 Strip Test and Direct Agglutination Test for Detection of Anti-Leishmania Donovani Antibodies in the Sudan
Background: Until now, the comparison of the rK39 strip test (RKT) and direct ag-glutination test (DAT) for detection of visceral leishmaniasis (VL) is exclusively based on either positive or negative qualification of the reaction outcome. Objective: In this study, we compared the diagnostic performance of RKT and DAT for VL both qualitatively and semi-quantitatively. Methods: For comparison ba...
متن کامل